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qd ngf  (Alomone Labs)


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    Alomone Labs qd ngf
    ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the <t>NGF</t> concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after <t>transfection,</t> <t>transport</t> of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.
    Qd Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qd+ngf/pmc05187584-143-6-14?v=Alomone+Labs
    Average 90 stars, based on 13 article reviews
    qd ngf - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands"

    Article Title: Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands

    Journal: Nature Communications

    doi: 10.1038/ncomms13865

    ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the NGF concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, transport of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.
    Figure Legend Snippet: ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the NGF concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, transport of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.

    Techniques Used: Sequencing, Binding Assay, Transfection, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction, Positive Control, Cell Culture, Concentration Assay, Fluorescence, Immunofluorescence, Imaging



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    qd ngf  (Alomone Labs)
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    Alomone Labs qd ngf
    ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the <t>NGF</t> concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after <t>transfection,</t> <t>transport</t> of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.
    Qd Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qd+ngf/pmc05187584-143-6-14?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    qd ngf - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    qd-ngfs  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology qd-ngfs
    ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the <t>NGF</t> concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after <t>transfection,</t> <t>transport</t> of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.
    Qd Ngfs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qd+ngf/pm19206333-256-5-33?v=Santa+Cruz+Biotechnology
    Average 90 stars, based on 1 article reviews
    qd-ngfs - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the NGF concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, transport of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.

    Journal: Nature Communications

    Article Title: Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands

    doi: 10.1038/ncomms13865

    Figure Lengend Snippet: ( a ) Partial sequence of the 3′-UTR of rat Pafah1b1 starting at the stop codon (*). The binding regions of the CUGU and control LNAs are indicated in maroon and grey, respectively. The CUGU element is underlined. ( b ) Dissociated DRG were transfected with control and CUGU LNA, and 24 h later, APC RNA immunoprecipitation was performed. Pafah1b1 was quantified by RT–PCR. 2 −ΔΔCT values are reported relative to Tubb3 (positive control, binds APC but is not targeted by the LNAs). Gfp was included as a control (no reads detected). Means±s.e.m. ( n =2 biological replicates with two technical replicates each). * P ≥0.05. t -test. ( c ) DRG neurons were cultured in microfluidic chambers. On DIV 3, the NGF concentration in the axonal chamber was changed to 5 ng ml −1 , and cell bodies were selectively transfected with the control or CUGU LNAs. Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 12 h, and axonal Pafah1b1 mRNA levels were determined by FISH. Background fluorescence was determined using a Gfp probe and subtracted. Means±s.e.m. of 15 optical fields per condition ( n =3 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's least significant difference test. Scale bar, 5 μm. ( d ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, axons were treated with 0, 5 or 100 ng ml −1 NGF for 10 min, and axonal Lis1 protein levels were measured by quantitative immunofluorescence. Means±s.e.m. of 20–30 optical fields per conditions ( n =4–6 biological replicates). * P ≥0.05. Two-way ANOVA with Fisher's LSD test. ( e – g ) DRG neurons were cultured and transfected as in a . Twenty-four hours after transfection, transport of LysoTracker-positive particles was observed in axons at baseline NGF ( e ), without NGF ( f ) or stimulated with NGF ( g ). Live-imaging time-lapse series of axonal fields were acquired, with images being taken every 13 s for 4 min. LysoTracker-positive particles with diameters ≥1 μm were scored as anterograde, retrograde, bidirectional or stationary. Means±s.e.m. of nine optical fields per conditions ( n =3 biological replicates). ** P ≥0.01. One-way ANOVA with Bonferroni's multiple comparisons test. NS, not significant.

    Article Snippet: For imaging transport of NGF-containing endosomes, QD-NGF was prepared by mixing mouse NGF 2.5S-Biotin (Alomone Labs, Jerusalem) and Qdot 585 Streptavidin Conjugate in a 1:1.2 molar ratio, and incubating them together at 4 °C with continuous inversion for 24 h. QD-NGF was diluted to 100 ng ml −1 and added to axons with a medium change 15 min before imaging.

    Techniques: Sequencing, Binding Assay, Transfection, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction, Positive Control, Cell Culture, Concentration Assay, Fluorescence, Immunofluorescence, Imaging